Fig. 2

1,25 vitamin D activates FBP1 to modulate AML Metabolism and block the “Warburg Effect” to enhance its anti-leukemic effect. A Table of RNA-seq results revealing that 1,25VD3 (80 nM) modulated different metabolic pathways in MV4–11 and MV4–11-MIDO-R cells by increasing gene expressions of certain enzymes related to gluconeogenesis, TCA cycles, oxidative phosphorylation, glycogenesis, and reducing gene expressions of certain enzymes related to glycolysis, glycogenolysis and nucleotide synthesis. B A summarized diagram. In addition to 1,25VD3’s known roles in inducing differentiation and inhibiting proliferation, we proposed a new functional role of vitamin D in the treatment of AML blasts. 1,25VD3 induces ~ 5000-fold increase of FBP1 (qPCR data) in AML blasts, which encodes large amounts of Fructose-1,6-bisphosphatase (extremely large bands in WB) to disrupt the progression of glycolysis and reduce the lactate production (Warburg Effect), a main energy resource for AML metabolism