Fig. 4

Effect of TEX41 silencing on cell cycle arrest. (A) Flow cytometry analysis of cell cycle distribution phases in 2′F-ANA Scramble (upper panel) and RS4;11 2′F-ANA-TEX41 (lower panel) RS4;11 treated cells for 72 h. The image is representative of three independent experiments with similar results. (B) The percentage of the cells in the G0-G1, S, and G2-M phase was reported as the mean of three independent experiments +/− SD. Western blot analyses of the indicated protein (C) and protein band quantifications (D) were performed in RS4;11 2′F-ANA Scramble and RS4;11 2′F-ANA-TEX41 treated cells for 72 h. Numbers represent molecular weight proteins expressed in kDa. ns = not significant; * = p-value < 0.05.