Fig. 3From: The lncRNA TEX41 is upregulated in pediatric B-Cells Acute Lymphoblastic Leukemia and it is necessary for leukemic cell growthEffect of TEX41 silencing on leukemic cell growth. (A) lncRNA TEX41 transcription levels in four B-ALL in vitro model systems (TOM-1, REH, SEM and RS4;11), AML in vitro model systems (NB4, HL-60, K562, OCI-AML-3), and PBMCs derived from 3 healthy subjects. The relative expression was determined using the 2−ΔCt method. TEX41 relative expression is shown as mean +/− SD of two technical independent experiments. (B) Bar-plot showing downregulation of TEX41 levels in RS4;11 cells treated with the 2′F-ANA oligonucleotides designed against lncRNA TEX41 and with 2′F-ANA Scramble for 48 h. The relative expression was determined using the 2−ΔCt method and it is shown as mean +/− SD of two technical independent experiments. (C) RS4;11 2′F-ANA Scramble (grey bars) and 2′F-ANA-TEX41 (red bars) treated cells growth (expressed as cells/µL). Cells concentrations are shown as mean +/− SD of three technical independent experiments. (D) Cyclins mRNA expression levels in RS4;11 2′F-ANA Scramble (grey bars) and RS4;11 2′F-ANA-TEX41 (red bar) treated cells. The relative expression was determined using the 2−ΔCt method. Cyclins relative expression is shown as mean +/− SD of two technical independent experiments. * = p-value < 0.05, ** = p < 0.01, Mann Whitney t-test.Back to article page