Fig. 2
Distributions of cell clusters and single-cell gene expression patterns of blast-like clusters between CN1 and CN2, CN2 and M2. A Overall t-distributed stochastic neighbor-embedding analysis of bone marrow mononuclear cells from the four samples (NC, CN1, CN2, M2). Clusters indicated by different colors and numbers; cell types indicated by different colors of loops. B Overall t-distributed stochastic neighbor-embedding analysis of bone marrow mononuclear cells from the four samples. Samples were indicated by different colors and numbers. C Volcano plot of differentially expressed genes (DEGs) in blast-like cells between CN1 and CN2. The dots on the left represent higher expressed genes in CN2 and those on right represent lower in CN2. D Metascape Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of enriched terms of DEGs in blast-like cell clusters between CN1 and CN2. Color shows p value. E Volcano plot of DEGs in blast-like cells between CN2 and M2. The dots on the left represent higher expressed genes in CN2 and those on right represent lower in CN2. F Metascape KEGG pathway analysis of enriched terms of DEGs in blast-like cell clusters between CN2 and M2. Color shows p value. G Heat map of DEGs among CN1, CN2, and M2 in blast-like cells. Yellow: higher expression level; red: lower expression level. Samples labeled in different colors. CN: Initial of the MDS-MF2 − 3 patient for single-cell sequencing; CN1: The patient CN at her MDS phase; CN2: The patient CN at her leukemic phase; NC: Normal control; M2: The patient with de novo AML-M2