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Fig. 2 | Biomarker Research

Fig. 2

From: Inhibition of BCL11B induces downregulation of PTK7 and results in growth retardation and apoptosis in T-cell acute lymphoblastic leukemia

Fig. 2

Downregulation of PTK7 induces cell growth retardation and apoptosis. (a) The BCL11B and PTK7 mRNA expression levels in T-ALL (Jurkat and Molt-4) and non-T-ALL (Raji) cell lines. GAPDH was used as an endogenous reference. (b) Cultured Jurkat and Raji cells were treated with Cy5-Sgc8 for 1 h at different concentrations. Flow cytometry analysis of the specific cell binding of Cy5-Sgc8 to Jurkat cells. (c) Expression of PTK7 in T-ALL cell lines 48 h after BCL11B siRNA transfection. (d) Effect of BCL11B siRNA on the BCL11B mRNA expression level by real-time quantitative polymerase chain reaction (qRT-PCR) 48 h after transfection in CB CD3+ T cells. Negative control siRNA-treated cells were used for comparison. (e) Knockdown efficiency in Molt-4 cells were analyzed after knocking down the PTK7 gene. (f) Molt-4 cells were transfected with si-PTK7 and then cell proliferation was assessed by Cell counting kit-8 (CCK-8) assay. (g) Apoptosis in Molt-4 cells transfected with si-PTK7 was measured by flow cytometry after 48 h. (h) Expression of the TRAIL and p27 genes in Molt-4 cells 48 h after transfected with BCL11B siRNA and PTK7 siRNA. Non-specific siRNA treated cells were used as negative control. Asterisks signify statistically significant differences (***, P < 0.001; **, P < 0.01; *, P < 0.05)

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