Fig. 5
From: Single-cell map of diverse immune phenotypes in the acute myeloid leukemia microenvironment
Unique CD4+ subsets revealed by scRNA-seq analysis. a UMAP plot of CD69highCD4+ population and CD69lowCD4+ population. These 2515 cells can be divided into 4 subsets. b Heatmap showing average expression level of cell-type genes expressed by the 4 clusters. c Histogram showed the fractions of different cell-type in CD69highCD4+ population and CD69lowCD4+ population for each AML patient and healthy donors’ BM cells, colored based on cell type. d The branched trajectory of state transition of naïve CD4+ T cells, TH17-like cells, and Treg cells in a two-dimensional state-space inferred by Monocle (version 2.14.0). Each dot corresponded to one single cell, colored according to its cluster label. e Expression maps showing log-normalized expression of typical markers (IL7R, IL2RA, MKI67, KLRB1, FOXP3, and CTLA4) in the differentiation of Naïve CD4+ T to TH17-like cells and/or Treg cells. Data are shown as log-normalized expression. Yellow indicates high expression, dark blue indicates low expression. f Violin plot showing the expression levels of functional genes (IGF1R, RORC, KLRB1, AHNAK, and IL10RA)