Fig. 2

Dissection and clustering of DC-like cells in AML patients and healthy donors. a UMAP plot of DC-like cells from Fig. 1a-represented DC cluster. These DC-like cells can be divided into 5 subsets. b Expression of DC-related genes across the transcriptionally defined DC clusters. c Dot plot of select canonical DC-related genes (cDC1: CLEC9A, ANPEP, and FBXO27; cDC2: CLEC12A, CLEC10A, SIRPA, and DENND3; pDC: IL3RA and JCHAIN) differentially expressed between different DC subsets. d Proportion of 5 DC subsets in total DC cells in each AML patient or healthy donor. Only samples of containing ≥10 DC-like cells were represented. e Dot plot of differentially expressed co-inhibitory and co-stimulation molecule genes. f Dot plot of differentially expressed cytokine genes. g The Kaplan-Meier overall survival curves of TCGA AML patients grouped by specific DC subset (CD206⁺ DC and CX3CR1⁺ DC) gene sets and DC-related genes (TGFB1, MRC1, CLEC7A, ITGAX, ITGB2, CX3CR1, CCL22, and TNFSF8). + represents censored observations, and P value was calculated by multivariate Cox regression