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Fig. 8 | Biomarker Research

Fig. 8

From: LncRNA APCDD1L-AS1 induces icotinib resistance by inhibition of EGFR autophagic degradation via the miR-1322/miR-1972/miR-324-3p-SIRT5 axis in lung adenocarcinoma

Fig. 8

Enhancement of icotinib sensitivity by APCDD1L-AS1 knockdown through promoting autophagic degradation of EGFR in xenograft mouse model. a-b The tumors isolated from nude mice subcutaneously injected with PC9/IcoRH/Lv-sh-APCDD1L-AS1 or PC9/IcoRH/Lv-NC cells were shown (a) (n = 6). Tumor growth curves were drawn according to the size detected per week (b). c-d The expression of APCDD1L-AS1, SIRT5 (c), miR-1322, miR-1972 and miR-324-3p (d) was analyzed by qRT-PCR assay. e The expression of SIRT5 and EGFR was determined by immunohistochemical staining (scale bar, 50 μm). f The LC3B punctures were observed by immunofluorescence (scale bar, 100 μm). The nuclei were visualized using DAPI. Representative images are presented on the figure. g The cell apoptosis of tumor sections was determined by TUNEL assay (scale bar, 100 μm). The mean gray value represented the status of cell apoptosis (Lv-NC group = 4.39 ± 0.568, Lv-shRNA group = 34.39 ± 2.273). The mean ± SD of triplicate experiments were plotted, **P < 0.01, ***P < 0.001

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Biomarker Research

ISSN: 2050-7771

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