Fig. 3
From: PSCA is a target of chimeric antigen receptor T cells in gastric cancer
Anti-PSCA CAR-T cells exhibited dramatic antitumor efficacy ex vivo. a. The lytic capacity towards different target cells, including BGC-823, KATO III and MKN-28 cells, was analyzed at the indicated effector-to-target ratios in a 24 h lysis assay. b. The concentrations of IL-2, IFN-γ, GM-CSF, and TNFα released by anti-PSCA CAR-T cells and GFP-T cells after coculture with BGC-823 cells overnight at an E:T ratio of 1:1 are shown. Error bars denote the s.e.m., and the results were calculated by an unpaired t test. * indicates p < 0.05; ** indicates p < 0.01; and *** indicates p < 0.001. c. Canonical T cell markers were detected by flow cytometry at a recommended E:T ratio of 1:1 after coculturing anti-PSCA CAR-T cells and GFP- T cells with target cell lines. d. Statistical analysis of three independent FACS results. Error bars denote the s.e.m.