Fig. 2
From: Recurrent SETD2 mutation in NPM1-mutated acute myeloid leukemia
SETD2 was required for the maintenance of NPM1-mutated AML cell line OCI-AML3. a and b The proliferation (a) and SETD2 expression (b) of scramble and SETD2 knockdown OCI-AML3 cells. c and d The proliferation (c) and SETD2 expression (d) of scramble and SETD2 knockdown THP-1 cells. e Annexin-V staining for detecting cell apoptosis in OCI-AML3 cells. f PI staining for cell cycle analysis in OCI-AML3 cells. g NPM1 has been demonstrated as one direct target of H3K36me3 in the literature report. h and i The expression of NPM1 (h) and its direct downstream targets, MESI and HOXA9 (i), was analyzed in scramble and SETD2 knockdown OCI-AML3 cells. ***, P < 0.001; **, P < 0.01; *, P < 0.05; T test was used for each graph