Fig. 1
From: CCR7 as a novel therapeutic target in t-cell PROLYMPHOCYTIC leukemia
T-PLL cells express high levels of functional CCR7 that is associated with shorter overall survival (OS). a CCR7 relative median fluorescence intensity (RMFI) in T-PLL cells compared to T-cells from healthy donors. T-cells obtained from PB samples of patients (n = 75) or healthy donors (n = 10) were analyzed by flow cytometry to determine CCR7 surface density measured as MFI relative to a corresponding IC. b Proportion of CCR7-positive T-cells is high in T-PLL. The percentage of CCR7-positive T-cells was determined in T-PLL patients (n = 109) and healthy donors (n = 14). In A-B the median ± interquartile range is shown. c CCR7 expression in pan T-cells or specific CCR7-expressing T-cell subsets (TN, TCM) from a representative patient and a healthy donor. The expression pattern and intensity of CCR7 is shown. d T-PLL cells migrate towards CCR7 ligands. Chemotaxis assays with T-PLL cells (n = 5) were performed with different doses of CCL19 and CCL21 (x axis). Each symbol represents the percentage of migrated cells in each experimental condition relative to the number of cells loaded in the upper chamber (% input). The mean ± SEM is shown. e CCR7 surface expression on T-PLL cells is associated with OS. The surface expression (% positive cells) of CCR7 was assessed by flow cytometry in T-PLL patients with clinical follow-up data (n = 96). At diagnosis, cases were classified as CCR7 positive (> 20% CCR7+ T-PLL cells) or negative (< 20%). OS was estimated and compared by Kaplan–Meier curves and log-rank test, respectively. The p-value of the log-rank statistical test is given. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant