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Fig. 1 | Biomarker Research

Fig. 1

From: Inducible transgene expression in PDX models in vivo identifies KLF4 as a therapeutic target for B-ALL

Fig. 1

Ectopic expression of KLF4 blocks EBV-mediated oncogenesis. A total of 2 × 105 normal naïve primary B-cells were infected with EBV in vitro to induce their transformation. B-cells were infected with a wildtype EBV strain (r_wt/B95.8 (6008), which contains all viral genes required for oncogenesis (mock) or with mutant EBV derivatives that additionally expressed either wildtype KLF4 (wtKLF4; 6948_EBNA2-T2A-KLF4) or a mutant derivative of KLF4 lacking its DNA-binding domain (mutKLF4; 6949_EBNA2-T2A-mutKLF4). After EBV infection, B-cells were harvested daily for up to 7 days. a Experimental design. b Capillary immunoassay of KLF4 2 days after infection with the indicated EBV viruses. β-Actin was used as a loading control. KLF4 expression was normalized to the loading control and was plotted as the fold change relative to that of the mock control. Representative result from two independent experiments are shown. c Cell numbers were quantified by flow cytometry (upper panel) and the fraction of apoptotic Annexin V binding cells was recorded (lower panel). The figure shows one experiment our of 3 replicates with B-lymphocytes obtained from three donors. d Cell cycle analysis: cells were incubated with 5-bromo-2′-deoxyuridine (BrdU) for 1 h prior to harvest and analyzed by flow cytometry after permeabilization and staining with a BrdU-specific antibody. The percentage of cells in the different phases of the cell cycle is indicated. See Supplementary Figure S3 for additional data

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