Fig. 2
From: Development of a highly sensitive method for detection of FLT3D835Y
Validation of the RFN-AS-PCR method by using mixtures of DNA samples from FLT3D835Y mutant and wild type AML blood samples. a Screening of 13 AML blood samples by using RFN-AS-PCR identified AML11 as a FLT3D835Y-positive case. b Verification of FLT3D835Y positivity in AML11 by Sanger sequencing. c and d Blood cell DNAs from AML11 and a patient with wild type FLT3 were mixed in the indicated proportions. AS-PCR was performed without EcoRV digestion (panel c) or with EcoRV digestion (panel d). Note that the EcoRV digestion increased the detection sensitivity from 1 to 0.001%