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Fig. 4 | Biomarker Research

Fig. 4

From: Chimeric antigen receptor T cells targeting PD-L1 suppress tumor growth

Fig. 4

CARPD-L1z T cells lysed PD-L1+ T cells. Percentage of PD-L1+ T cells in a CD4+ T cells (gated on CD3+CD8 cells) and b CD8+ T cells (gated on CD3+CD8+ cells) post activated by CD3 and CD28 antibodies. FACS detection of PD-L1 expression at indicated time points. Percentage of PD-L1+ T cells in c CD4+ CARMSLNz T cells (gated on CD3+GFP+CD4+ cells) and d CD8+ CARMSLNz T cells (gated on CD3+GFP+CD8+ cells) post co-cultured with H460-MSLNGL cells. CARMSLNz T cells were co-cultured with H460-MSLNGL cells for 0 h, 16 h, 24 h, 40 h and 48 h at a definitive E: T ratio (1: 1), then the expression of PD-L1 was detected by FACS. e Percentage of PD-L1+ T cells in CAR19z T and CARPD-L1z T cells (gated on CD3+GFP+ cells) post activated by CD3 and CD28 antibodies for 16 h. f Percentage of PD-L1+ T cells in CAR19z T cells (gated on CD3+GFP+ cells) post co-cultured with NALM6 cells for 24 h at a definitive E: T ratio (2: 1), and percentage of PD-L1+ T cells in CARPD-L1z T cells (gated on CD3+GFP+ cells) post co-cultured with H460GL cells for 24 h at a definitive E: T ratio (2: 1). g Percentage of PD-L1+ T cells in CAR19BBz T cells (gated on CD3+GFP+ cells) post co-cultured with or without NALM6 cells. CAR19BBz T cells were co-cultured with or without NALM6 cells for 12 h, at a definitive E: T ratio (2: 1), then the expression of PD-L1 was detected by FACS. h Percentage of PD-L1+ T cells in CARMSLNz T cells (gated on CD3+GFP+ cells) post co-cultured with H460-MSLNGL cells with or without AZ (20 μg/mL) for 24 h at a definitive E: T ratio (1: 1). i Percentage of CD25+CD69+ T cells in CARPD-L1z T and CARMSLNz T cell (gated on GFP+ cells) post co-cultured with uPD-L1 T cells at a definitive E: T ratio (1: 1) for 24 h. j Percentage of CD19+ T cells (uPD-L1 T cells) after co-cultured with CARPD-L1z T or CARMSLNz T cell at a definitive E: T ratio (1: 1) for 24 h (gated on live cells). k The production of IL-2 and IFN-γ of CARPD-L1z T or CARMSLNz T cells post co-culture with uPD-L1 T cells for 24 h at a definitive E: T ratio (1: 1). Error bars denote SD, and the results were compared by unpaired t-test. * P < 0.05, ** P < 0.01, and *** P < 0.001

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