Fig. 3

Combined CARMSLNz T with CARPD-L1z T or dPD1z T cells failed to achieve a synergistic anti-tumor effect in vivo. a Schematic diagram of the CAR construction of CARMSLNz. b In vitro killing of CARPD-L1z T, CARMSLNz T, the combination of CARMSLNz T and CARPD-L1z T and CAR19z T cells against H460-MSLNGL cell line. Each type of cell was co-cultured with H460-MSLNGL cell line at the indicated effector to target (E: T) ratios for 24 h, and the luciferase activities were calculated to determine the percentages of cytolysis. c In vitro killing of dPD1z T, CARMSLNz T, the combination of CARMSLNz T and dPD1z T and CAR19z T cells against H460-MSLNGL cell line. Each type of cell was co-cultured with H460-MSLNGL cell line at the indicated effector to target (E: T) ratios for 24 h, and the luciferase activities were calculated to determine the percentages of cytolysis. d Tumor volumes and e tumor weights of NSCLC PDX (P4) after treatment with CARPD-L1z T, CARMSLNz T, the combination of CARMSLNz T and CARPD-L1z T or CAR19z T cells. NSI mice were transplanted with primary NSCLC cells at day 0, subsequently, CAR T (5 × 10⁶) cells were infused on day 10. Tumor volumes were monitored at indicated days and tumor weights were measured after mice euthanasia. The result of tumor volume represent mean ± SEM, and was compared by two-way ANOVA with Tukey’s multiple comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001. The result of tumor weight represent mean ± SD, and was compared by unpaired t-test. * P < 0.05, and ** P < 0.01. f Percentages of CAR T cells in the spleen after treated with each type of CAR T cells (gated on live cells) are shown. Error bars denote SD, and the results were compared by unpaired t-test. * P < 0.05. g Tumor volumes and h tumor weights of NSCLC PDX (P4) after treatment with dPD1z T, CARMSLNz T, the combination of CARMSLNz T and dPD1z T or CAR19z T cells. NSI mice were transplanted with primary NSCLC cells at day 0, subsequently, CAR T (5 × 10⁶) cells were infused twice at day 10 and day 20. Tumor volumes were monitored at indicated days and tumor weights were measured after mice euthanasia. The result of tumor volume represent mean ± SEM, and was compared by two-way ANOVA with Tukey’s multiple comparisons test. ** P < 0.01, and *** P < 0.001. The result of tumor weight represent mean ± SD, and was compared by unpaired t-test. * P < 0.05, ** P < 0.01, and *** P < 0.001