Fig. 2
From: Chimeric antigen receptor T cells targeting PD-L1 suppress tumor growth
dPD1z T and CARPD-L1z T inhibited the growth of multiple types of PD-L1+ tumors in vivo. a Tumor volumes and b tumor weights of H460GL cells xenograft mice after treatment with CARPD-L1z T, dPD1z T, CAR19z T cells or untreated controls (Blank). NSI mice were transplanted with H460GL cells (5 × 105) at day 0, subsequently, CARPD-L1z T, dPD1z T or CAR19z T (5 × 106) cells were infused at day 10. Tumor volumes were monitored at indicated days and tumor weights were measured after mice euthanasia. The result of tumor volume represent mean ± SEM, and was compared by two-way ANOVA with Tukey’s multiple comparisons test. ** P < 0.01, and *** P < 0.001. The result of tumor weight represent mean ± SD, and was compared by unpaired t-test. * P < 0.05, ** P < 0.01, and *** P < 0.001. c Tumor volumes and d tumor weights of NSCLC PDX mice after treatment with dPD1z T, CAR19z T cells or untreated controls (Blank). NSI mice were transplanted with primary NSCLC cells at day 0, subsequently, dPD1z T or CAR19z T (5 × 106) cells were infused twice at day 15 and day 20. Tumor volumes were monitored at indicated days and tumor weights were measured after mice euthanasia. The result of tumor volume represent mean ± SEM, and was compared by two-way ANOVA with Tukey’s multiple comparisons test. ** P < 0.01. The result of tumor weight represent mean ± SD, and was compared by unpaired t-test. * P < 0.05. e Percentages of GFP+ T cells in peripheral blood (PB), spleen, and tumors after treated with dPD1z T or CAR19z T cells (gated on live cells) are shown. Error bars denote SD, and the results were compared by unpaired t-test. * P < 0.05, ** P < 0.01, and *** P < 0.001. f Primary and secondary tumor images of gastric cancer PDX models after treatment with dPD1z T, CAR19z T cells or untreated controls (blank). g Primary tumor weights and h spleen weights of gastric cancer PDX after treatment with dPD1z T, CAR19z T cells or untreated controls (Blank). NSI mice were transplanted with primary gastric cancer cells at day 0, subsequently, dPD1z T or CAR19z T (5 × 106) cells were infused twice at day 20 and day 24. Tumor weights and spleen weights were measured at day 45 after mice euthanasia. The results represent mean ± SD, and were compared by unpaired t-test. * P < 0.05, ** P < 0.01, and *** P < 0.001. i Percentages of T cells in peripheral blood (PB), spleen, and tumors after treated with dPD1z T or CAR19z T cells (gated on live cells) are shown. Error bars denote SD, and the results were compared by unpaired t-test. * P < 0.05, and ** P < 0.01