Fig. 4
Tan-CAR-transduced T cells effectively clear tumors in vivo. (a) Schematic of the xenograft model used to investigate the activity of tan-CAR T cells in vivo. NSG mice were injected with 7 × 106 K562-transduced luciferase-positive target cells via the tail vein; bioluminescent imaging was performed on day 7 and every 4 days thereafter. CAR T cells (107) or NC T cells (107) were provided by intravenous injection on days 8 and 10. (b) Bioluminescence radiance was used as a surrogate marker for tumor burden. (c) Time course of tumor growth based on mouse whole-body bioluminescence. The mean signal per mouse ± SD is as shown. Statistical analysis was performed using day 19 data (the last time point at which a sufficient number of mice that did not receive treatments remained viable) using one-way ANOVA followed by Tukey’s multiple comparisons test. The data are presented as the mean ± SD (n = 3); ***p < 0.001