Fig. 1

Generation and characterization of tan-CAR T cells. (a) Schematic representation of the plasmid design used to construct single and tandem scFv-CAR-modified T cells. The tan-CAR construct encodes a tandem fusion of the BCMA-scFv and CD19-scFv domains, a CD28 costimulatory domain, and a CD3 zeta-mediated activation signaling domain. (b) Analysis of transduction efficiency. Isolated and activated human T cells were transduced with the lentiviral vectors CD19-CAR, BCMA-CAR, or tan-CAR. Transduction efficiency was evaluated by flow cytometry using fluorescent-tagged protein L to detect variable light chain Ig together with human CD19 and human BCMA protein. (c) Quantitative evaluation of CD4⁺/CD8⁺ T-cell ratios. CAR-positive CD4⁺ T cells and CAR-positive CD8⁺ T cells were detected by flow cytometry. Data are representative of three donors