Table 1 Glossary of TIS terminology
Glossary of TIS terms | |
|---|---|
toponome | the spatial network of proteins within intact cells |
TIS | toponome imaging system – a robotically-controlled serial immunostainer |
CMP | combinatorial molecular phenotype- a designation that summarizes the presence [1] or absence (0) of all markers in a given pixel (i.e. 1000110100011) |
binarization | selecting of a threshold in aligned, background-subtracted images of immuno-fluorescence above which a marker is considered present [1] and below which it is absent (0) |
.xml file | a data file merging all of the binarization data from each marker into a single file and showing the CMP present in every pixel of an image |
triplets | CMPs that are present in all three subjects in each experimental group in the 50 most frequent/abundant CMPs in the xml files. |
2-of-3 | CMPs that are present in 2 out of the 3 subjects in each group in the 50 most frequent/abundant CMPs in the xml files |