Fig. 7

Effects of CDK1 overexpression and downregulation on megakaryocytic polyploidization in cells overexpressing DLGAP1. a Immunofluorescent microscopy of UT7/TPO cells overexpressing GFP-DLGAP1 and transfected with constitutively active clone of CDK1 (Flag-CDK1-AF). GFP-DLGAP1 was detected green, the constitutively active CDK1 clone was stained red with anti-Flag antibody and DNA was stained blue with DAPI. b Immunofluorescent microscopy of K562 cells overexpressing GFP-DLGAP1 and constitutively active clone of CDK1(Flag-CDK1-AF). The GFP-DLGAP1 was captured using green fluorescence, the constitutively active CDK1 clone was stained red with anti-Flag antibody) and DNA was stained blue with DAPI. c Immunofluorescent microscopy of K562 cells overexpressing GFP-DLGAP1 and dominant negative clone of CDK1(Flag-CDK1-ND). The GFP-DLGAP1 was captured using green fluorescence, the dominant negative CDK1 clone was stained red with anti-Flag antibodyand DNA was stained blue with DAPI