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Fig. 1 | Biomarker Research

Fig. 1

From: DLGAP1 directs megakaryocytic growth and differentiation in an MPL dependent manner in hematopoietic cells

Fig. 1

Insertional mutagenesis screening system for MPLsignaling cooperating gene factors. a Left panel represents schematic of the inducible MPL signaling system comprising the F36 V-MPL fusion construct that upon addition of the AP20187 drug leads to the MPL domain dimerization and transactivation followed by downstream MPL signaling processes. Right panel shows schematics of the MGIFMNOo and MFHuMIGNOo constructs used in functional screens in K562 and UT7/TPO cell lines. b Line graphs of K562 and UT7 cells selection on AP20187 after MGIFMNOo transduction. c Bar graph of 4 days treatment of plain and MPL driven K562 cells with AG490. The horizontal line at 20 × 10^3 represents the number of seeded cells

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