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Table 1 Classification and counts for the sequencing results

From: Identification of TCR Vβ11-2-1-1-1 T cell clone specific for WT1 peptides using high-throughput TCRβ gene sequencing

Sample Read pairs Effective reads Unique Unique Unique VDJ Unique CDR3aa
Control 5227607 4297968 62 14 1499 17789
Cytokines 5110352 3254496 64 14 1448 13828
WT1 6591869 4968087 62 14 1437 14472
B3A2 5854623 3542234 64 14 1393 11747
  1. Control: the untreated group; Cytokines: cells cultured with IL-2 and Il-7 in vitro for 21 days; WT1: cells treated with WT1 peptide (RMFPNAPYL, AA 126–134) and cultured with IL-2 and IL-7 in vitro for 21 days; B3A2: cells treated with 6 different BCR-ABL peptides and cultured with IL-2 and IL-7 in vitro for 21 days; An effective read is a read possessing the full CDR3 structure; Unique : types of gene usage; Unique : types of gene usage; Unique VDJ: unique combinations of V/D/J genes; Unique CDR3aa: unique CDR3 amino acid sequences