Fig. 2

Effect of IGF1 treatment on the transcription of stemness marker genes. Cells were treated or not with 1 μg/ml IGF1 after 24 h in the serum/supplement-free medium. Cells were harvested in RNA extraction buffer 48 h after the treatment. qPCR performed with GAPDH serving as internal housekeeping control. Fold change in gene expression was calculated by ΔΔCt method. Fold change in untreated controls was considered as 1 by convention. Relative fold changes compared between untreated (control) and treated cells were plotted for all the indicated cell types. Significance (p < 0.05) was indicated by asterisk after performing one way ANOVA. a NBMEL: newborn melanocytes; b. SKMEL28: melanoma; c patient C76N; d patient 139N; e patient PD1N