Skip to main content

Advertisement

Table 2 Methylation summary of whole genome bisulphite sequencing

From: Reduced nuclear DNA methylation and mitochondrial transcript changes in adenomas do not associate with mtDNA methylation

  Normal mucosa (n = 5) Adenoma (n = 4)
Nuclear Genome
CG methylation % 74.5 ± 0.50 67.9 ± 1.16*
CHG methylation % 1.3 ± 0.06 1.0 ± 0.05
CHH methylation % 1.3 ± 0.07 1.0 ± 0.05
Mitochondrial Genome
CG methylation % 1.0 ± 0.09 1.0 ± 0.16
CHG methylation % 1.3 ± 0.14 0.8 ± 0.14
CHH methylation % 1.2 ± 0.06 0.92 ± 0.12
Lambda control % unconverted cytosines 1.06 ± 0.06 0.86 ± 0.05
  1. Average methylation percentage ± SEM, *P < 0.01 Normal vs Adenoma. No statistical tests were performed on methylation levels within the range of the lambda controls (which indicate conversion efficiency)