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Fig. 5 | Biomarker Research

Fig. 5

From: The orphan nuclear receptor EAR-2 (NR2F6) inhibits hematopoietic cell differentiation and induces myeloid dysplasia in vivo

Fig. 5

Inhibition of differentiation is DNA-binding and HDAC dependent. a Transduction of 32D cells with an EAR-2 mutant that could not bind DNA or with the HDAC inhibitor b sodium butyrate rescued ability of EAR-2 to inhibit differentiation. c Transfection of EAR-2 showed repression of luciferase activity, d that could be de-repressed with HDAC inhibitors. e Ability of known nuclear receptor ligands to activate luciferase activity was tested using a hybrid construct containing the EAR-2 ligand binding domain. This was contrasted with a hybrid construct containing the PPARɣ ligand binding domain. (* indicates p < 0.05 compared to negative control)

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