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Table 3 Protein identification

From: Platelet protein biomarker panel for ovarian cancer diagnosis

Protein spot number on 2D Gene ontology name Protein name theoretical experimental NCBI accession number Sequence coverage, % Matched peptides Total peptide count score msms
pI Mr, kDa pI Mr, kDa
1 2 3 4 5 6 7 8 9 10 11 12 13
285 ACTN1 Alpha-actinin-1 isoform b 5.2 104 5.5 100 NP_001093 25 19 75 98
1836 ACTN4 Alpha actinin 4, partial 5.2 74 5.3 72 AAH15620 14 9 30 68
1153 ACTB beta actin variant, partial 5.4 42 6.2 40 BAD96752.1 31 9 48 71
1803 ACTB actin beta 5.2 39 5.3 42 BAG62914.1 30 10 54 81
1772 AP4A Bis(5′-nucleosyl)- tetraphosphatase 5.1 17 5.4 17 NP_001152 27 5 36 52 1
1274 CAPZA1 capping actin protein of muscle Z-line alpha subunit 1 6 32 5.4 40 XP_005542394.1 54 11 60 106
911 CD41 Integrin alpha-IIb precursor variant 5.7 60 5.4 60 BAD92789.1 46 1
276 CD41 Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41), isoform CRA_a 5.4 104 5.3 110 EAW51594.1 24 17 49 122
1761 CD42A glycoprotein IX platelet 5.4 18 5.4 20 AAH30229 27 5 43 53 2
792 CD61 Platelet glycoprotein IIIa, partial 5 87 5.6 70 AAA52600 15 1
942 CNDP2 Cytosolic non-specific dipeptidase 5.6 53 6 55 BAG53426 22 8 48 51 3
1539 CRKL Crk-lke protein 6.3 34 6.5 26 NP_005198 29 9 56 64 1
1525 ERP29 Erp28, endoplasmic reticulum resident protein 29 isoform 1 precursor 6.8 29 6.5 27 NP_006808 28 6 68 44 2
1854 FBG Fibrinogen gamma chain 5.5 48 5.6 50 EAX04921 40 13 53 113
397 GELS Gelsolin isoform a precursor (782 aa) 5.9 86 5.4 95 NP_000168 61 1
401 GELS Gelsolin isoform b (731 aa) 5.6 81 5.7 90 NP_937895 12 9 50 45 2
311 HSPA9 Stress-70 potein, mitochondrial precursor 5.8 74 5.7 120 NP_001126860 27 17 66 100 3
1155 HP Haptoglobin 6.3 38 5.4 42 AAI07588.1 25 10 61 71
482 HSPA8 Heat shock 70 kDa protein 1A/1B 5.4 70 5.7 80 P08107 13 6 56 29
1115 ILEU Leukocyte elastase inhibitor 5.9 43 6.3 43 NP_109591 21 7 43 72
1177 MPST 3-mercaptopyruvate sulfurtransferase 6.8 35 6.4 37 BAD92061.1 33 8 39 75
1231 NSFL1C NSFL1 cofactor p47 isoform X8 5 31 6.2 38 XP_011527607.1 25 5 46 38 1
1850 PGM1 phosphoglucomutase-1 isoform 1 6.3 62 6.8 70 NP_002624.2 11 5 32 33
1464 PHB Prohibitin 5.5 28 5.7 30 XP_003912755.3 49 12 46 136
1054 RNH1 ribonuclease/angiogenin inhibitor 1 4.8 50 4.8 50 AAH11186.1 11 5 25 45
999 PRKAR1A protein kinase cAMP-dependent type I regulatory subunit alpha 5.2 43 5.5 53 XP_004041145.1 35 13 72 102  
939 PRKAR2B protein kinase cAMP-dependent type II regulatory subunit beta 5 45 4.8 55 BAG54705.1 19 6 27 41 2
1175 SRB6 Serpin B6 4.2 39 5.6 40 XP_011512978.1 34 10 45 76
1821 SRC Protooncogene tyrosine-protein kinase Src 7.1 60 6.6 60 NP_005408.1 13 8 54 39
1801 TUBA4A Tubulin alpha-4A chain 4.9 49 5.1 55 NP_001265481.1 23 10 56 73
1801 TUBA1C tubulin alpha 1c 4.9 59 5.1 55 BAH11541.1 23 10 56 68  
758 TLN1 Talin 1 5.8 272 5.9 70 AAF27330 7 13 43 42 1
755 TLN1 Talin 1 5.8 272 5.8 70 AAF27330 9 15 46 54
292 TLN1 Talin 1 5.8 272 5.8 110 AAF27330 14 33 84 106
774 TLN1 Talin 1 5.8 272 6.4 70 AAF27330 25 1
1409 TLN1 Talin 1 5.8 272 6.4 34 AAF23322.1 6 15 39 47
1640 TPM1 Tropomyosin 1, alpha 5 20 4.7 20 XP_005254707 23 6 64 30
1894 TUBA6 Tubulin alpha-6 chain, partial 5 47 5.3 56 EHH66249.1 34 10 56 80
1894 TUBA8 tubulin alpha-8 5 43 5.3 56 XP_007469555.1 33 10 56 82  
1894 TUBA1C tubulin alpha-1C chain 5 47 5.3 56 XP_004646666.1 35 10 56 81  
999 TUBB1 tubulin beta-1 chain isoform X7 5.1 43 5.5 53 XP_018872841.1 42 12 72 93
1850 WDR1 WD repeat-containing protein 1 iso 1 6.4 59 6.8 70 AAD05045 35 18 60 135
  1. 1 - number of a protein spot on 2-D gel,
  2. 2 - gene ontology name,
  3. 3 - protein name,
  4. 4 - isoelectric point of a protein spots according to the position on 2D gel,
  5. 5 - protein mass of a protein according to the position of a spot on 2D gel,
  6. 6 - isoelectric point of a protein spot as provided by Mascot search database,
  7. 7 - protein mass as provided by Mascot search database,
  8. 8 - protein accession number according to NCBI,
  9. 9 - matching of the experimental peptide sequence to a peptide sequence provided by NCBI,
  10. 10 - number of identified peptides that matched to the peptides of a peptide sequence provided by NCBI,
  11. 11 - total amount of peptides in a peptide sequence provided by NCBI,
  12. 12 - score provided by Mascot search engine,
  13. 13 - performed msms for the certainty of protein identification.