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Table 3 Protein identification

From: Platelet protein biomarker panel for ovarian cancer diagnosis

Protein spot number on 2D

Gene ontology name

Protein name

theoretical

experimental

NCBI accession number

Sequence coverage, %

Matched peptides

Total peptide count

score

msms

pI

Mr, kDa

pI

Mr, kDa

1

2

3

4

5

6

7

8

9

10

11

12

13

285

ACTN1

Alpha-actinin-1 isoform b

5.2

104

5.5

100

NP_001093

25

19

75

98

1836

ACTN4

Alpha actinin 4, partial

5.2

74

5.3

72

AAH15620

14

9

30

68

1153

ACTB

beta actin variant, partial

5.4

42

6.2

40

BAD96752.1

31

9

48

71

1803

ACTB

actin beta

5.2

39

5.3

42

BAG62914.1

30

10

54

81

1772

AP4A

Bis(5′-nucleosyl)- tetraphosphatase

5.1

17

5.4

17

NP_001152

27

5

36

52

1

1274

CAPZA1

capping actin protein of muscle Z-line alpha subunit 1

6

32

5.4

40

XP_005542394.1

54

11

60

106

911

CD41

Integrin alpha-IIb precursor variant

5.7

60

5.4

60

BAD92789.1

46

1

276

CD41

Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41), isoform CRA_a

5.4

104

5.3

110

EAW51594.1

24

17

49

122

1761

CD42A

glycoprotein IX platelet

5.4

18

5.4

20

AAH30229

27

5

43

53

2

792

CD61

Platelet glycoprotein IIIa, partial

5

87

5.6

70

AAA52600

15

1

942

CNDP2

Cytosolic non-specific dipeptidase

5.6

53

6

55

BAG53426

22

8

48

51

3

1539

CRKL

Crk-lke protein

6.3

34

6.5

26

NP_005198

29

9

56

64

1

1525

ERP29

Erp28, endoplasmic reticulum resident protein 29 isoform 1 precursor

6.8

29

6.5

27

NP_006808

28

6

68

44

2

1854

FBG

Fibrinogen gamma chain

5.5

48

5.6

50

EAX04921

40

13

53

113

397

GELS

Gelsolin isoform a precursor (782 aa)

5.9

86

5.4

95

NP_000168

61

1

401

GELS

Gelsolin isoform b (731 aa)

5.6

81

5.7

90

NP_937895

12

9

50

45

2

311

HSPA9

Stress-70 potein, mitochondrial precursor

5.8

74

5.7

120

NP_001126860

27

17

66

100

3

1155

HP

Haptoglobin

6.3

38

5.4

42

AAI07588.1

25

10

61

71

482

HSPA8

Heat shock 70 kDa protein 1A/1B

5.4

70

5.7

80

P08107

13

6

56

29

1115

ILEU

Leukocyte elastase inhibitor

5.9

43

6.3

43

NP_109591

21

7

43

72

1177

MPST

3-mercaptopyruvate sulfurtransferase

6.8

35

6.4

37

BAD92061.1

33

8

39

75

1231

NSFL1C

NSFL1 cofactor p47 isoform X8

5

31

6.2

38

XP_011527607.1

25

5

46

38

1

1850

PGM1

phosphoglucomutase-1 isoform 1

6.3

62

6.8

70

NP_002624.2

11

5

32

33

1464

PHB

Prohibitin

5.5

28

5.7

30

XP_003912755.3

49

12

46

136

1054

RNH1

ribonuclease/angiogenin inhibitor 1

4.8

50

4.8

50

AAH11186.1

11

5

25

45

999

PRKAR1A

protein kinase cAMP-dependent type I regulatory subunit alpha

5.2

43

5.5

53

XP_004041145.1

35

13

72

102

 

939

PRKAR2B

protein kinase cAMP-dependent type II regulatory subunit beta

5

45

4.8

55

BAG54705.1

19

6

27

41

2

1175

SRB6

Serpin B6

4.2

39

5.6

40

XP_011512978.1

34

10

45

76

1821

SRC

Protooncogene tyrosine-protein kinase Src

7.1

60

6.6

60

NP_005408.1

13

8

54

39

1801

TUBA4A

Tubulin alpha-4A chain

4.9

49

5.1

55

NP_001265481.1

23

10

56

73

1801

TUBA1C

tubulin alpha 1c

4.9

59

5.1

55

BAH11541.1

23

10

56

68

 

758

TLN1

Talin 1

5.8

272

5.9

70

AAF27330

7

13

43

42

1

755

TLN1

Talin 1

5.8

272

5.8

70

AAF27330

9

15

46

54

292

TLN1

Talin 1

5.8

272

5.8

110

AAF27330

14

33

84

106

774

TLN1

Talin 1

5.8

272

6.4

70

AAF27330

25

1

1409

TLN1

Talin 1

5.8

272

6.4

34

AAF23322.1

6

15

39

47

1640

TPM1

Tropomyosin 1, alpha

5

20

4.7

20

XP_005254707

23

6

64

30

1894

TUBA6

Tubulin alpha-6 chain, partial

5

47

5.3

56

EHH66249.1

34

10

56

80

1894

TUBA8

tubulin alpha-8

5

43

5.3

56

XP_007469555.1

33

10

56

82

 

1894

TUBA1C

tubulin alpha-1C chain

5

47

5.3

56

XP_004646666.1

35

10

56

81

 

999

TUBB1

tubulin beta-1 chain isoform X7

5.1

43

5.5

53

XP_018872841.1

42

12

72

93

1850

WDR1

WD repeat-containing protein 1 iso 1

6.4

59

6.8

70

AAD05045

35

18

60

135

  1. 1 - number of a protein spot on 2-D gel,
  2. 2 - gene ontology name,
  3. 3 - protein name,
  4. 4 - isoelectric point of a protein spots according to the position on 2D gel,
  5. 5 - protein mass of a protein according to the position of a spot on 2D gel,
  6. 6 - isoelectric point of a protein spot as provided by Mascot search database,
  7. 7 - protein mass as provided by Mascot search database,
  8. 8 - protein accession number according to NCBI,
  9. 9 - matching of the experimental peptide sequence to a peptide sequence provided by NCBI,
  10. 10 - number of identified peptides that matched to the peptides of a peptide sequence provided by NCBI,
  11. 11 - total amount of peptides in a peptide sequence provided by NCBI,
  12. 12 - score provided by Mascot search engine,
  13. 13 - performed msms for the certainty of protein identification.