Clinical significance of intronic variants in BRAF inhibitor resistant melanomas with altered BRAF transcript splicing

Table 1 Predicted effects of −51 mutation in intron 8

Data derived using the Human Splice Finder Tool [12]
Branch Site score above 67 is considered a potential break point, and score variation between wild type and mutant sequence of less than −10% is considered to break the branch point
Splice Site values above 65 are predicted splice sites, the −51 G mutation is underlined in Cryptic Donor Site
Splicing factor SRSF6 motif with highest predicted scores is shown [12]
Motif position is relative to first nucleotide of exon 9

ISSN: 2050-7771