Fig. 1

BRAF intronic splice and branch point sequences in melanomas expressing the BRAF exon 4–8∆ transcript. a Schematic diagram of the BRAF gene, showing exons (numbered boxes) and introns (black bars) and the alternate splicing event leading to the BRAF exon 4–8∆ transcript. b Sanger sequencing traces showing the sequence encompassing the junction between BRAF intron 8 and exon 9 in BRAF inhibitor resistant melanomas (Progs) expressing the BRAF exon 4–8∆ transcripts and derived from patient 7, 10 and 28. The branch point motifs, including the −51 nucleotide associated with BRAF exon 4–8∆ splicing, and splice sites are boxed