Fig. 5

Measurement of hexokinase activity from normal and breast cancer serum after AlbuVoid™ enrichment and PEP protein separation and elution (120 min). The conditions were the same as in Fig. 4, hexokinase activity was measured by NADP reduction at 340 nm at 0 and 120 min, the OD340 nm increase from 120 min. was calculated by subtracting the value from the 0 min. reading. Boxed fractions indicated samples of interest