Fig. 4

Measurement of hexokinase activity from normal and breast cancer serum after AlbuVoid™ enrichment and PEP protein separation and elution (60 min). 800 μg AlbuVoid-enriched serum proteins from normal group was loaded onto an IPG strip and separated by IEF. After further separation by SDS-PAGE, the proteins were eluted into the PEP plate and hexokinase activities were analyzed from each of the 384 wells. Hexokinase activity was measured by NADP reduction at 340 nm at 0 and 60 min, the OD340 nm increase from 60 min. was calculated by subtracting the value from the 0 min. reading. Boxed fractions indicated samples of interest