Fig. 5

Time-dependent Hexokinase Activity Analysis from AlbuVoid-enriched Lung Cancer Patient Serum. 750 μg AlbuVoid-enriched serum proteins from lung cancer patient group was loaded onto an IPG strip and separated by IEF. After further separation by SDS-PAGE, the proteins were eluted into the PEP plate and hexokinase activities were analyzed from each of the 384 wells. Hexokinase activity was measured by NADP reduction at 340 nm at 0 (a), 60 min (b). and overnight (c) incubation