Fig. 2From: Identification of functional metabolic biomarkers from lung cancer patient serum using PEP technologyEffective 2-D Gel Separation of Beef Liver Proteins without Reducing Reagent. 400 μg of beef liver protein was loaded on an 11 cm pH 3-10Nonlinear IPG strip, for the second dimension, the proteins were separated in SDS-PAGE with gradient gel of 10–20 %. After fixing, the proteins were detected by Sypro Ruby stainingBack to article page