Fig. 1

Enrichment of Low Abundance Proteins from Human Serum. 800 μl pooled serum from normal (N = 20) and lung cancer patient (N = 20) was used for the enrichment of low abundance proteins with AlbuVoid treatment according to the manufacturer’s instruction. For the comparison, 750 μg protein from untreated serum (normal group) and treated serum (normal group) was loaded onto two 11 cm IPG strips for IEF protein separation without reducing reagent. For second dimension separation, SDS-PAGE with 10–20 % gradient gel was used, Sypro Ruby was used for protein staining and detection