Table 1 Oligonucleotide primers used for PCR
From: Suspected anemia caused by maternal anti-Jra antibodies: a case report
PCR (target) | Primer name | Nucleotide sequence (5′–3′) |
|---|---|---|
JRA337/376/1515-SSP | ||
PCR-1 (Exon 13) | JRA1515-F4 | AAGTCAAAGGCAGATGCTTC |
PCR-2 (Exon 13) | JRA1515-R2 | CCTTATCAGAGCAAACAGAG |
PCR-3 (Exon 4) | JRA376-F4 | CCAAGTGGATTATCTGGAGATG |
PCR-4 (Exon 4) | JRA376-R6 | TTGTCTCCTTTGTCTTTTACCAAACCCACTAATACTTTCTTG |
PCR-5 (Exon 4) | JRA376-R1 | CAAACCCACTAATACTTACTTA |
PCR-6 (Exon 4) | JRA337-R10 | TACATTTGAAATTGCCAGGTCA |
PCR-7 (Exon 4) | GPC4-3 | TGTGGCATTGTATCTTGTCCT |
PCR-8 (Exon 4) | GPC4-4 | GTGGCTATCTCGGGAAGAAT |
JRA34/421-SSP | ||
PCR-9 (Exon 2) | JRA34-F2 | TGGTATGGGCCATTCATTG |
PCR-10 (Exon 2) | JRA34-R7 | CATTGGTGTTGCCTCGTGACAT |
PCR-11 (Exon 2) | JRA34-R10 | CTTCGACAGCGCCCCTTCGGATTGGTGTTGCCTCGTGACAC |
PCR-12 (Exon 5) | JRA421-F4 | CTGACAGTGAGAGAAAACTTAA |
PCR-13 (Exon 5) | JRA421-15 | ACTACAACACTACCCGTGAGTGACGGTGAGCGAAAACTTAC |
PCR-14 (Exon 5) | JRA421-R2 | CACTTTATCCAGACCTAACTC-3′ |