Fig. 4

The effect of triptolide on reef coral red and GFP proteins expressions in A549 lung tumor cells. The undamaged or cisplatin-damaged pDsRed2-C1 plasmid (pDsRed2-C1-Cis*) was co-transfected with pmaxGFP plasmid DNA into A549 cells. The transfected cells were cultured in the presence or absence of triptolide (10 ng/ml) for 24 h and expressions of both reef coral red and GFP proteins were detected by fluorescence microscope using excitation/emission lights with wavelengths of 563 nm/582 nm and 475 nm/505 nm respectively for reef coral red and GFP proteins. The light image of the same view was also documented for visualization of the live cells