Fig. 3From: Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISHDetection of BCR-ABL T315I mutation by PNA. Detection of BCR-ABL T315I mutation by PNA (green signal) in CD34+ cells enriched from CML patients carrying T315I mutation. Panel a and b show T315I positive cells from a patient with 60Â % of mutated cells positive cells, panel c and d show T315I positive cells from a patient with 45Â % of mutated cells. Negative control (e, f) is represented by a CML patient without T315I mutation. No specific PNA green signal can be detected in the absence of the mutationBack to article page