Fig. 1From: Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISHExperimental design: perfect PNA/DNA hybridization occurs when template sequence is w.t., thus leading to suppression of PCR amplification. By contrast, when in presence of single base-pair mismatch (i.e. T315I, indicated by x), PNA/DNA duplex is strongly destabilized allowing template amplification. Empty and filled arrows represent DNA primers used for PCR amplification and PNA competitor, respectivelyBack to article page