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Figure 3 | Biomarker Research

Figure 3

From: Effects of Chinese herbal medicine in combination with mitomycin C on gastric cancer cells

Figure 3

Effect of pre-treated with MMC followed by exposure to BZYQT on induction of apoptosis. (A) MKN-74 cells were treated with 0.25 or 1.0 μg/ml MMC for 24 h. Subsequently, cells were washed and treated with or without 0.82 mg/ml of BZYQT for 48 h. Cells were counted and scored for apoptoic chromatin condensation by the quantitative fluorescence microscopy. Bars represent mean ± SD of cells with apoptosis counted as a percentage of 400 total cells randomly counted in duplicate samples. **P <0.01 compared with untreated control group. #P < 0.01 compared each other. (B) Photomicrographs of representative fields of MKN-74 cells stained with bisbenzimide trihydrochloride (Hoescht-33258) to evaluate nuclear chromatin condensation after treatment with no drug (panel A), 1.0 μg/ml MMC for 24 h then cells were washed followed by incubation with no drug for 48 h (panel B), 1.0 μg/ml MMC for 24 h then cells were washed followed by exposure to 0.82 mg/ml of BZYQT for 48 h (panel C), no drug for 48 h prior to 50 μM safingol combined with 1.0 μg/ml MMC for 24 h (panel D).

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