Figure 2

Effect of combination BZYQT with MMC on induction of apoptosis. MKN-74 cells were treated with 0.82 mg/ml of BZYQT for 48 h. Then 0.25 or 1.0 μg/ml MMC was added for another 24 h. (A) Cells were counted and scored for apoptoic chromatin condensation by the quantitative fluorescence microscopy. Bars represent mean ± SD of cells with apoptosis counted as a percentage of 400 total cells randomly counted in duplicate samples. (B) Apoptotic cell death was evaluated by quantitative by DNA fragmentation by using single-strand DNA ELISA kit. Values are expressed as mean ± SD from three separate experiments.