Production of HGF in co-cultures of BMSC and MM cells. BMSC or HS-5 cells were seeded at a concentration of 3 × 104 cells per well (0.5 mL) into 24 well plastic plates and 2 – 6 × 104 myeloma cells or the non-myeloma cell line Jurkat were added. Supernatants were harvested after 72 h of incubation. The levels of HGF were measured by enzyme-linked immunosorbent assay. (A) BMSC co-cultured with 6 × 104 (ANBL-6, U266, INA-6, IH-1, OH-2 or the non-myeloma cell line Jurkat) or 2 × 104 (JJN3) myeloma cells. (B) HS-5 cells co-cultured with 6 × 104 ANBL-6 cells or 2 × 104 JJN3 cells. (C) BMSC were seeded into inlet of 24 transwell plastic plates and 6 × 104 ANBL-6 cells or 2 × 104 JJN3 cells per well (0.1 mL) were added to the lower compartment of the transwell. Cultures were maintained as described above, supernatants were harvested after 72 h, and the levels of HGF were measured by enzyme-linked immunosorbent assay.