Table 5 Methylation studies on DNA extracted from HCC blood samples
From: DNA methylation: potential biomarker in Hepatocellular Carcinoma
With clinicopathological correlation | ||||||
Marker genes | HCC patients (n) | Clinicopathological correlation | Validation method | Samples used for DNA extraction | Publication | Year |
DAPK, p16 | 64 | AFP level | MSP | Serum | Lin et al. [104] | 2005 |
RASSF1A | 40 | Tumor size | MSP | Plasma | Yeo et al. [106] | 2005 |
LINE-1 | 85 | OS, tumor size | COBRA | Serum | Tangkijvanich et al. [109] | 2007 |
RASSF1A | 85 | DFS | Methylscreen | Serum | Chan et al. [107] | 2008 |
CCND2 | 70 | DFS | qMSP | Serum | Tsutsui et al. [105] | 2010 |
APC, DKK, DLC1, CDH1, RUNX3, SFRP1, WIF1 | 108 | CIMP + associated with gender, HBV infection, AFP level, tumor stage, DFS | MSP | Plasma | Liu et al. [101] | 2011 |
APC, GSTP1, RASSF1A, SFRP1 | 72 | OS (APC, RASSF1A) | Methylscreen | Plasma | Huang et al. [108] | 2011 |
LINE-1 | 305 | Increased risk of HCC | Pyrosequencing | White blood cells | Wu et al. [110] | 2012 |
IGFBP7 | 136 | Vascular invasion | MSP | Serum | Li et al. [115] | 2013 |
XPO4 | 44 | AFP level | MSP | PBMC | Zhang et al. [114] | 2013 |
TFPI2 | 43 | Tumor stage | MSP | Serum | Sun et al. [113] | 2013 |
APC | 23 | Portal vein thrombosis | qMSP | Serum | Nishida et al. [112] | 2013 |
Without clinicopathological correlation | ||||||
Marker genes | HCC patients (n) | Clinicopathological correlation | Validation method | Samples used for DNA extraction | Publication | Year |
p16 | 29 | NIL | qMSP | Serum and buffy coat | Wong et al. [103] | 2003 |
p16 | 46 | NIL | MSP | Serum | Chu et al. [121] | 2004 |
GSTP1 | 32 | NIL | MSP | Serum | Wang et al. [122] | 2006 |
CDH1, p16, RASSF1A, RUNX3 | 8 | NIL | MSP | Serum | Tan et al. [123] | 2007 |
p16, p15, RASSF1A | 50 | NIL | MSP | Serum | Zhang et al. [124] | 2007 |
GSTP1, RASSF1A | 26 | NIL | MSP | Serum | Chang et al. [125] | 2008 |
RASSF1A | 35 | NIL | MSP | Serum | Hu et al. [126] | 2010 |
APC, CDH1, FHIT, p15, p16 | 28 | NIL | MSP | Plasma | Iyer et al. [127] | 2010 |
DBX2, THY1 | 31 | NIL | Bisulfite seq | PBMC | Zhang et al. [41] | 2013 |