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Table 5 Methylation studies on DNA extracted from HCC blood samples

From: DNA methylation: potential biomarker in Hepatocellular Carcinoma

With clinicopathological correlation
Marker genes HCC patients (n) Clinicopathological correlation Validation method Samples used for DNA extraction Publication Year
DAPK, p16 64 AFP level MSP Serum Lin et al. [104] 2005
RASSF1A 40 Tumor size MSP Plasma Yeo et al. [106] 2005
LINE-1 85 OS, tumor size COBRA Serum Tangkijvanich et al. [109] 2007
RASSF1A 85 DFS Methylscreen Serum Chan et al. [107] 2008
CCND2 70 DFS qMSP Serum Tsutsui et al. [105] 2010
APC, DKK, DLC1, CDH1, RUNX3, SFRP1, WIF1 108 CIMP + associated with gender, HBV infection, AFP level, tumor stage, DFS MSP Plasma Liu et al. [101] 2011
APC, GSTP1, RASSF1A, SFRP1 72 OS (APC, RASSF1A) Methylscreen Plasma Huang et al. [108] 2011
LINE-1 305 Increased risk of HCC Pyrosequencing White blood cells Wu et al. [110] 2012
IGFBP7 136 Vascular invasion MSP Serum Li et al. [115] 2013
XPO4 44 AFP level MSP PBMC Zhang et al. [114] 2013
TFPI2 43 Tumor stage MSP Serum Sun et al. [113] 2013
APC 23 Portal vein thrombosis qMSP Serum Nishida et al. [112] 2013
Without clinicopathological correlation
Marker genes HCC patients (n) Clinicopathological correlation Validation method Samples used for DNA extraction Publication Year
p16 29 NIL qMSP Serum and buffy coat Wong et al. [103] 2003
p16 46 NIL MSP Serum Chu et al. [121] 2004
GSTP1 32 NIL MSP Serum Wang et al. [122] 2006
CDH1, p16, RASSF1A, RUNX3 8 NIL MSP Serum Tan et al. [123] 2007
p16, p15, RASSF1A 50 NIL MSP Serum Zhang et al. [124] 2007
GSTP1, RASSF1A 26 NIL MSP Serum Chang et al. [125] 2008
RASSF1A 35 NIL MSP Serum Hu et al. [126] 2010
APC, CDH1, FHIT, p15, p16 28 NIL MSP Plasma Iyer et al. [127] 2010
DBX2, THY1 31 NIL Bisulfite seq PBMC Zhang et al. [41] 2013
  1. AFP, Alpha fetoprotein; COBRA, Combined Bisulfite Restriction Analysis; DFS, Disease free survival; HBV, Hepatitis B virus; MSP, Methylation-specific PCR; qMSP, Quantitative MSP; OS, Overall survival; PBMC, Peripheral blood mononuclear cells; Seq, Sequencing.