DNA methylation: potential biomarker in Hepatocellular Carcinoma

Biomarker Research

Table 3 Commonly studied methylation markers in HCC

Gene	HCC patients (n)	Clinicopathological correlation	Validation method	Publication	Year
p16	28	Tumor stage	MSP	Yu et al. [56]	2003
	18	Tumor stage	MSP	Shim et al. [59]	2003
	20	Tumor differentiation	MSP	Qin et al. [66]	2004
	50	Age, gender, virus infection (HBV/HCV)	MSP	Li et al. [62]	2004
	44	HBV infection	MSP	Jicai et al. [63]	2006
	60	Age, tumor stage, vascular invasion, virus infection (HBV/HCV)	MSP	Katoh et al. [61]	2006
	58	Tumor stage	MSP	Su et al. [60]	2007
	23	HBV infection (HBx)	MSP	Zhu et al. [65]	2007
	265	Disease free survival	MSP	Ko et al. [70]	2008
	118	Gender	Methylscreen	Wang et al. [119]	2012
SOCS1	50	Liver cirrhosis	MSP	Okochi et al. [77]	2003
	51	HCV infection	MSP	Yang et al. [54]	2003
	284	Age, tumor size, virus infection (HBV/HCV)	MSP	Ko et al. [73]	2008
	77	HCV infection	COBRA	Nishida et al. [76]	2008
	46	Liver cirrhosis, tumor size	MSP	Chu et al. [75]	2010
	46	Tumor stage	MethyLight	Um et al. [72]	2011
	29	Chemotherapy treatment	MSP	Saelee et al. [120]	2012
	116	Age and gender	Methylscreen	Zhang et al. [74]	2013
GSTP1	60	Overall survival	MSP	Lee et al. [55]	2003
	83	Alcohol consumption, gender	MSP	Zhang et al. [82]	2005
	60	Gender, viral infection (HBV/HCV)	MSP	Katoh et al. [61]	2006
	58	HBV infection, tumor stage	MSP	Su et al. [60]	2007
	77	HCV infection	COBRA	Nishida et al. [76]	2008
	166	HBV infection	Pyrosequencing	Lambert et al. [81]	2011
CDH1	60	Overall survival	MSP	Lee et al. [55]	2003
	32	Vascular invasion, recurrence	MSP	Ghee [91]	2005

COBRA, Combined Bisulfite Restriction Analysis; HBV, Hepatitis B virus; HCV, Hepatitis C virus; HBx, Hepatitis B virus X protein; MSP, Methylation-specific PCR.

ISSN: 2050-7771