The three subclones from the ascites cytospin sample of a progressive epithelial ovarian cancer patient. BAC probes containing the c-myc, Rb1, Chk2, p53 or BRCA1 genes were labeled with Spectrum Green, PF555 (red), PF590 (orange), HyPer5 (purple) or PF415 (blue), respectively. The mixed probes were hybridized with the ascites cytospin sample from a progressive epithelial ovarian cancer patient. The results revealed that there were three subclones showing distinct combinations of signal patterns for the five selected genes. The details of the molecular profiling are shown in Table 1.