Figure 2From: Development of TAP, a non-invasive test for qualitative and quantitative measurements of biomarkers from the skin surface Effects of different printing buffers on the detection of human IgG printed on nitrocellulose. Various amounts of human IgG (1.2, 0.6, 0.3, 0.15 and 0.075 ng /spot) were printed on Whatman Protran BA-85 (0.45 μm) membrane using various printing buffers. Printed IgG was visualised in spot-ELISA and signals quantified by determining the pixel intensities of digitized spots. Panel A: Each line on graph represents the analyses results of printed IgG in different printing buffer consisting of PBS + 10% glycerol supplemented with either 0.1% or 0.05% Triton X-100, 0.5% Tween-20, 1% ethanol or nothing, or printing buffer consisting of PBS + 20% glycerol, supplemented with either 0.1% or 0.05% Triton X-100, 0.5% Tween-20, 1% ethanol or nothing (see Panel A for details). Each data point consists of measurements of five spots on five different strips (N = 25 per data point). X-axis: human IgG amount per spot. Y-axis: Staining intensity defined as the mean pixel intensity measured on a 0–255 grey scale. Panel B: R2 and CV (Coefficient of variation) value presented for each tested printing buffer.Back to article page