Figure 1

PTEN-null pancreas (transversal and coronal sections). Typical pseudo-color images of the redox indices and their corresponding histograms of a pancreas in the PTEN-null group. The top two rows are the transversal section with a milling depth of 400 μm and the bottom two rows are the coronal section with a milling depth 340 μm. The mean values and SDs are shown on each histogram. From left to right: Fp nominal concentration (μM), NADH nominal concentration (μM), Fp redox ratio, Fp/NADH, and NADH/Fp. The color bars of the Fp and NADH images indicate the nominal concentrations in μM. The color bars of the Fp redox ratio images indicates the ratio range from 0-1. The x-y axes of the pseudo-color images are the dimension of scan matrix, representing the physical location of the tissue relative to the origin of the fiberoptic probe. The x axes of the corresponding histograms represent the Fp or NADH concentration or their ratios. The y axes represent the number of pixels in the tissue section having a specific value of fluorophore concentration or ratio. Image resolution: 100 μm.