Figure 2

Detection of 2-DE separated ITIH4 fragment on nitrocellulose membrane. Neat serum samples were subjected to 2-DE, western blotting and detection with enzyme-conjugated CGB lectin. Panel (a) demonstrates a typical profile of healthy non-pregnant women controls, while panels (b) and (c) are representative profiles of healthy pregnant women and patients with hydatidiform mole, respectively. The cleavage fragment of ITIH4 (ITIH4f) appears to be overexpressed in all subjects’ profiles relative to the controls. For all panels, the acidic side of the blot is to the left and relative molecular mass declines from the top.